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Direct sputum microscopy performed at Designated Microscopy Centres (DMC) is a relatively low-risk activity as long as safe work practices are implemented properly.

The following work practices are recommended to ensure that microscopy laboratory technicians are not exposed to aerosols from sputum specimens.

1. Administrative Control at DMC

  • The layout and design of the DMCs should include natural ventilation, mechanical ventilation (exhaust fan) and unidirectional airflow.
  • The sputum collection area should be separated from the laboratory.
  • Provisions for biomedical waste management in deep burial pits, sharps pits or appropriate disposal via the health facility biomedical waste management system.

2. Environment Control at DMC 

  • Doors and windows should be kept open for air exchanges.
  • Sputum must be collected in a well-ventilated area with direct sunlight. It should not be collected inside the laboratories, toilets, waiting rooms, reception rooms, or any other enclosed space.

3. Personnel Level Control at DMC 

  • Laboratory technicians should wear laboratory coats, gloves and masks when handling sputum containers and during smear preparation and staining.
  • Smears should be prepared in a well-ventilated environment near an open flame.
  • Work benches should be cleaned daily with disinfectants.
  • Sputum containers, applicator/ broomsticks, and used slides should be disinfected with 5% Phenol overnight before discarding.

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Question Answer 1 Answer 2 Answer 3 All Correct answer Correct explanation Page id Part of Pre-test Part of Post-test

Sputum specimens can be collected in toilets.

 

True False     2 Sputum must be collected in a well-ventilated area with direct sunlight. It should not be collected inside the laboratories, toilets, waiting rooms, reception rooms, or any other enclosed space.   Yes Yes

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