LPA Biosafety Essentials: Procedural Biosafety Measures

Procedural biosafety measures include: 

• Biosafety measures taken during the Line Probe Assay (LPA) procedures ​ 
• Aerosol generation and preventive measures​ 

Manipulations that produce aerosols in DNA Extraction Room are: 

• Pipetting​ 
• Centrifugation​ 
• Vortexing​ 
• Discarding micropipette tips after use​ 

Measures to Prevent Aerosol Generation ​ 

• Always open and manipulate specimens and cultures in a certified Biosafety Cabinet (BSC)​. 
• Work over absorbent material (sheet/ paper) soaked in a disinfectant​.
• Use centrifuge safety cups/ aerosol tight rotor and open them in the BSC​. 

Resources 

• Tuberculosis Laboratory Biosafety Manual. 

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