Interpretation of SL-LPA: Predicting Drug resistance in Special Cases

Notes

• Bands with intensities similar or better than Amplification Control (AC) zone are considered.
• Not all bands of strip show same signal strength

Special Scenarios

1. Both mutation probe and corresponding wild type probe are developed, represents a valid result
   • Possible reasons:
     • The tested specimen contains hetero-resistant strain
     • The tested specimen contains more than one M. tuberculosis strain (mixed infection)
2. Resistance with wild type pattern
   • Possible reasons:
     • The tested specimen contains hetero-resistance strain; caused by mutation not covered by mutation probes
     • The tested specimen contains the wild type and resistant strain (mixed infection); mutation not covered by mutation probes
3. Complete gene locus (all bands including Locus Control band) missing, invalid result
   • Possible reason:
     • DNA concentration below the limit of detection

Resources

• GenoType MTBDRsl VER 2.0 kit, instructions for use

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