LPA Quality Assurance: Cleaning of Lab

General Instructions

• Dedicated cleaning supplies, brooms required for different areas.
• Cleaning should be done by trained Polymerase Chain Reaction (PCR) laboratory staff, not by general cleaning personnel.
• Meticulous cleaning of the work area, surfaces, racks, pipettes and instruments should be done before and after performing each procedure with disinfectants (freshly prepared 1% sodium hypochlorite, followed by 70% alcohol).
• Use dedicated spray flasks/ containers for disinfectants.
• Clean paper towels (not cloth) should be used to decontaminate surfaces and equipment.
• Walls should have high-gloss enamel/ gloss-based paint for easy cleaning.
• Floors must be covered with vinyl, linoleum or ceramic tiles.
• Bench-tops must be made of impermeable melamine to eliminate crevices.
• Instruments should be cleaned and maintained daily/ weekly as described in the equipment training content.
• Cleaning and maintenance log sheets of all areas should be maintained.

Area-wise Cleaning

Weekly meticulous cleaning once; clean top-to-bottom, starting with bench tops and finishing with mopping the floor with disinfectants.

Area 1: DNA extraction room

Clean and maintain instruments (pipettes, biosafety cabinet, centrifuge and water bath) daily/ weekly.

Area 2: Pre-amplification room

Clean and maintain instruments (pipettes, PCR equipment) daily/ weekly.

Area 3: Amplification room

Clean and maintain instruments (thermal cycler, pipettes, PCR equipment) daily/ weekly.

Area 4: Hybridization room

Clean and maintain instruments (GT-Blot, Twincubator, pipettes, GT-Blot hybridization machine) daily/ weekly.

Resources

• Line Probe Assays for Drug-resistant Tuberculosis Detection, GLI.
• Molecular Detection of Drug-resistant Tuberculosis by Line Probe Assay, FIND.

Kindly provide your valuable feedback on the page to the link provided HERE