Work-up for Positive MGIT Cultures

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Work-up for Positive MGIT Cultures

Learning Objectives

Describe process from tube removal to purity check

Content

The different steps of tube removal are as follows:

1. Visual Observation:
 Characteristic flake-like growth (Presumptive Positive)
 Uniform Turbidity (Presumptive Contamination)

2. Smear:
 Corded AFB smear-positive (Culture positive)
 Both Acid Fast or Non-Acid Fast Bacilli (Culture with contamination)
 Only Non-Acid Fast Bacilli (Contamination)

3. Inoculate LJ slant/BHI for contamination check by the rate of growth, morphology, smear and immunochromatographic assays

 Growth in 24 – 48 hours (Contamination)

 No Growth (Contamination free)

 Cultures should be checked for purity and identification of M. tuberculosis using a rapid immunochromatographic species identification test, such as MPT 64 Ag Assay

Resource

Mycobacteriology Laboratory Manual

Assessment

Question 1	Answer 1	Answer 2	Answer 3	Answer 4	Correct Answer	Correct Explanation	Page id	Part of Pre-Test	Part of Post-Test
AFB smear should be prepared from a positive MGIT tube.	True	False			True	AFB smear should be prepared from a positive MGIT tube.		Yes	Yes
Question 2
Sub-culturing a positive MGIT tube on an LJ slant is recommended.	True	False			True	Sub-culturing a positive MGIT tube on an LJ slant is recommended.		Yes	Yes

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Work-up for Positive MGIT Cultures

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Work-up for Positive MGIT Cultures

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