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CDST_LT-M13: LC-DST

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  3. CDST_LT-M13: LC-DST
  4. CDST_LT-M13: LC-DST

  • CDST_LT: LT DST Introduction

    • MTB Drug Resistance
    • Mechanism of action-anti TB drugs first line and second line
    • Drug Susceptibility Methods

  • CDST_LT: Requirements for submission, acceptance and processing of isolates for DST

    • Requirements for submission, acceptance and processing for culture Isolates
    • Acceptance of MTB Isolates for DST
    • Preparing the Isolate for DST
    • Preparing the Isolate for Liquid Culture DST

  • CDST_LT: SIRE susceptibility testing

    • SIRE Susceptibility Testing
    • Critical Concentrations and Reconstitution of SIRE Drugs

  • CDST_LT: Pyrazinamide (PZA) susceptibility testing

    • Pyrazinamide PZA Susceptibility Testing
    • Critical Concentration and Reconstitution of PZA

  • CDST_LT: Second line drug testing

    • Second-line Phenotypic Drug Susceptibility Testing
    • Critical Concentration and formulations of Second-line Anti-TB Drugs for DST
    • Preparation and Storage of Second-line Anti-TB Drugs used for DST

  • CDST_LT: Inoculum preparation and incubation for MGIT DST

    • Inoculum Preparation for First and Second-line Phenotypic DST
    • Inoculum Preparation from MGIT Positive Culture
    • Inoculation of Growth Control Tube/Drug Containing Tubes
    • PZA Inoculum Preparation
    • Inoculation of PZA Growth Control/Drug Containing Tube
    • Incubation of MGIT 960 DST Sets

  • CDST_LT: Reading and reporting of MGIT 960 DST results

    • Principle of MGIT DST Reading
    • Procedure for Reading MGIT DST Results
    • Unloaded MGIT DST Report
    • Validation of Resistant/Unexpected MGIT DST Results
    • LC DST Recording and Reporting
    • Tuberculosis Laboratory Register
    • Drug Susceptibility Testing Logs
    • Final Result Reporting

  • CDST_LT: MGIT DST quality control

    • MGIT DST Quality Control
    • Standard Controls
    • Procedure of Quality Control
    • Quality Control of the MGIT DST Inoculum
    • Quality Control in DST results interpretation

  • CDST_LT: Quality assurance of TB Culture & DST laboratories

    • Quality Assurance in TB C&DST Laboratories
    • C&DST Laboratory Arrangement and Administration
    • Pre-examination QA Procedures in C&DST Laboratories
    • Examination QA Procedures in C&DST Laboratories
    • Post-examination QA Procedures in C&DST Laboratories
    • Quality Improvement Activities in C&DST Laboratories
    • External Quality Assurance for C&DST laboratories

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